Fig. 6.

Viperin is an important ISG to control ZIKV infection in cortical neurons. Primary cortical neurons isolated from WT and viperin^−/− mice were infected with WNV (a), JEV (b), or ZIKV (c) (MOI, 0.1), and viral growth was determined by focus-forming assay at indicated time points. Subsets of neurons were treated with 5000 U/ml IFNαB/D for 16 h prior to infection. Statistical significance was calculated using unpaired t test, significance is indicated by asterisks (*P < 0.05, **P < 0.01). The results are cumulative of at least two independent experiments performed in triplicates