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Steady-state kinetic parameters of Cupriavidus taiwanensis tryptophan hydroxylase W192F (CtAAAH-F) and variants produced by semi-rational evolution
| Km (mM) | kcat (s−1) | Vmax (mM · s−1) | Relative kcat/Km | |
|---|---|---|---|---|
| CtAAAAH-F | 3.0 | 0.4 | 1.2 | 0.13 |
| CtAAAAH-F197I | 2.1 | 1.3 | 2.7 | 0.62 |
| CtAAAAH-F197 L | 1.6 | 1.1 | 1.8 | 0.69 |
| CtAAAAH-E219C | 1.8 | 1.5 | 2.7 | 0.83 |
| CtAAAH-LC | 0.95 | 1.9 | 1.8 | 2.00 |
