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. 2018 Mar 16;13(3):e0194150. doi: 10.1371/journal.pone.0194150

Fig 1. Designing and construction of pBI::HAR1.

Fig 1

Primer extension PCR was done with a pair of overlapping primers, HAR-F & HAR-R (S1 Table) containing XbaI and SacI recognition sequences at their 5′ ends. XbaI and SacI digested PCR product was ligated onto pUC19. pBI121 backbone was generated by digestion with XbaI and SacI that released GUS coding sequence. After sequence confirmation, HaAce1-preamiRNA1 DNA fragment was released from recombinant pUC19 and ligated onto pBI121 backbone to get pBI::HAR1.