Table 1. Sequences of primers and protospacer sequence of gRNAs used in this work.
| Name | Sequence (5' - 3') |
|---|---|
| pUC57-T7-prom-fw | GATCCCTAATACGACTCACTATA |
| pUC57-sgRNA-rv | AAAAAAAGCACCGACTCG |
| C-dv2-16681-start-fw | CAATATGCTGAAACGCGAGAGAAAC |
| P2A-rv | AGGTCCAGGGTTCTCCTCCACG |
| hCMV-DV216681+8-fw-Gib | AGAGCTCGTTTAGTGAACCGAGTTGTTAGTCTACGTGGACCGACAAAGACAG |
| C-dv2-16681-start-rv | GTTTCTCTCGCGTTTCAGCATATTG |
| P2A-C-16681-fw | CGTGGAGGAGAACCCTGGACCTATGAATAACCAACGGAAAAAGGCG |
| E-dv2-16681-end-rv | CCTGCACCATGACTCCCAAATAC |
| E-dv2-16681-end-fw | GTATTTGGGAGTCATGGTGCAGG |
| 6685_6709_rv_den2 | CCAGTATTATTGAAGCTGCTATCCA |
| DV2-16681-6695_6718-fw | CTTCAATAATACTGGAGTTTTTTCTCATAG |
| 3’ UTR-DV2 | AGAACCTGTTGATTCAACAGCACCATTCCATTTTCTGGCGTTCTGTGCCTGGAATGAT |
| HDV-fw-dv2 | CTGTTGAATCAACAGGTTCTGGGTCGGCATGGCATCTCC |
| CMV-5’ UTR-DV2 | CGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCG |
| C25-GFP-fw | CGAGAGAAACCGCGTGTCGACTATGGTGAGCAAGGGCGAGGAG |
| P2A-GFP-rv | CAGGCTGAAGTTAGTAGCTCCGCTTCCCTTGTACAGCTCGTCCATGCCG |
| pUC57-T7-prom-fw | GATCCCTAATACGACTCACTATA |
| and pUC57-sgRNA-rv | AAAAAAAGCACCGACTCG |
| C25-bfloGFPa1-fw | CGAGAGAAACCGCGTGTCGACTATGCCTCTGCCCGCAACCCACG |
| P2A-bfloGFPa1-rv | CAGGCTGAAGTTAGTAGCTCCGCTTCCATGGTGATGGTGATGGTGAGCC |
| C25-GFP-fw | CGAGAGAAACCGCGTGTCGACTATGGTGAGCAAGGGCGAGGAG |
| P2A-GFP-rv | CAGGCTGAAGTTAGTAGCTCCGCTTCCCTTGTACAGCTCGTCCATGCCG |
| NS4B-16681-end-fw | GAAGAACACAACCAACACAAGAAGG |
| C25-16681-rv | AGTCGACACGCGGTTTCTCTCGC |
| C25-C26-16681-fw | GAGAAACCGCGTGTCGACTGTGCAACAGCTGACAAAGAGATTCTCAC |
| NS4B-16681-end-rv | CCTTCTTGTGTTGGTTGTGTTCTTC |
| T2A-clover-fw |
GGAAGCGGAGAGGGCAGAGGAAGTCTGCTAACATGCGGTGACGTCGAGGAGAATCCTGGACCTATGGTGAGCAAGGGCGA GGAGCTGTTCA |
| T2A-C25-rv |
AGGTCCAGGATTCTCCTCGACGTCACCGCATGTTAGCAGACTTCCTCTGCCCTCTCCGCTTCCAGTCGACACGCGGTTTC TCTCGCGTTTC |
| T2A-GFP-fw | GACGTCGAGGAGAATCCTGGACCTATGGTGAGCAAGGGCGAGGAG |
| P2A-GFP-rv | CAGGCTGAAGTTAGTAGCTCCGCTTCCCTTGTACAGCTCGTCCATGCCG |
| T2A-bfloGFPa1-fw | TGCGGTGACGTCGAGGAGAATCCTGGACCTATGCCTCTGCCCGCAACCCACG |
| P2A-bfloGFPa1-rv | CAGGCTGAAGTTAGTAGCTCCGCTTCCAGCCAGCTCGTAGAACGCCTTCTC |
| T2A-mTSaph-tdT-mAmet-fw | TGCGGTGACGTCGAGGAGAATCCTGGACCTATGGTGAGCAAGGGCGAGGAG |
| P2A-mCardinal-mNeptune2-rv | CAGGCTGAAGTTAGTAGCTCCGCTTCCCTTGTACAGCTCGTCCATGCCATT |
| T2A-mNeptune2-fw | TGCGGTGACGTCGAGGAGAATCCTGGACCTATGGTGTCTAAGGGCGAAGAGCTGA |
| P2A-mCardinal-mNeptune2-rv | CAGGCTGAAGTTAGTAGCTCCGCTTCCCTTGTACAGCTCGTCCATGCCATT |
| P2A-tdTomato-mAmet-rv | CAGGCTGAAGTTAGTAGCTCCGCTTCCCTTGTACAGCTCGTCCATGCCGT |
| T2A-LSS-mKate2-fw | TGCGGTGACGTCGAGGAGAATCCTGGACCTATGAGCGAGCTGATTAAGGAGAACATG |
| P2A-LSSmKate2-rv | CAGGCTGAAGTTAGTAGCTCCGCTTCCATTAAGCTTGTGCCCCAGTTTGCTAGG |
| T2A-miRFP703-fw | TGCGGTGACGTCGAGGAGAATCCTGGACCTATGGTAGCAGGTCATGCCTCTGGC |
| P2A-miRFP703-rv | CAGGCTGAAGTTAGTAGCTCCGCTTCCGCTCTCAAGCGCGGTGATCCG |
| 5UTR-DV2-16681-fw | AGTTGTTAGTCTACGTGGACCGACAAAG |
| C-end-16681-rv | CGCCATCACTGTTGGAATCAGCATAATG |
| gRNA-C25-16681 | GAGAAACCGCGTGTCGACTA |
| gRNA-eGFP-P2A | GACGAGCTGTACAAGGGAAG |
| gRNA-T2A-Clover2 | ATGGTGAGCAAGGGCGAGGA |
| gRNA-Clover-eGFP-P2A | CTTCCCTTGTACAGCTCGTC |