Fig 2. Fabs CB017.5 and CB002.5 bind with high affinity to RSV G and a G peptide.
Surface plasmon resonance (SPR) response curves of Fab CB002.5 (top) and Fab CB017.5 (bottom) binding to wild-type RSV sG from strain A2 (A) and a subtype A RSV G peptide encompassing the central conserved region (B). The raw data are plotted in black, and the calculated best fit to a 1:1 binding model is plotted in red. The equilibrium dissociation constant (KD) for each interaction is displayed above the respective SPR curve. (C) Sequence alignment of the 45-residue G peptide and the corresponding region of RSV G from strains A2 and B1. The strictly conserved residues, the cystine noose, and CX3C motif are labeled.