Figure 3. Effect of ginsenoside Rg1 on the AMPK pathway. The high-fat diet (HFD)-fed mice were treated with or without ginsenoside Rg1 at a dose of 300 mg·kg^-1·day^-1 (Rg1-300) or 500 mg·kg^-1·day^-1 (Rg1-500) for 2 months (n=12 mice/group). Immunoblotting assays detected the basal protein and phosphorylation levels of AMPK in liver (A) and muscle (B). Results are representative of 2 of 12 mice per group (upper panels). The quantified data are shown in the lower panels. ND: normal diet. Data are reported as means±SD. ***P<0.001, vs HFD group (ANOVA).