Table 2. Quantification of total phenolic compounds, total flavonoids and tannins and antioxidant properties (total antioxidant capacity, TAC), reducing power, DPPH radical-scavenging effect and ORAC assay of melon peel and seed extracts.
Bioactive compounds and antioxidant activities | Melon peel extracts (dry extract) | Melon seed extracts (dry extract) | ||||
---|---|---|---|---|---|---|
Aqueous | Hydromethanolic (70:30 v/v) | Hydroethanolic (70:30 v/v) | Aqueous | Hydromethanolic (70:30 v/v) | Hydroethanolic (70:30 v/v) | |
Total phenols compounds (mg GAE/100 g) | 110.7±15.03c | 703.1±12.65a | 203.1±7.17b | 75.2±7.39b | 111.7±12.36a | 69.77±12.78b |
Total flavonoids content (µg CE/100 g) | 262±0.20b | 125±0.10a | 104±0.15c | 109±0.40a | 93±0.20a | 99±0.30a |
Tannins (µg CE/100 g) | nd | 309±0.20a | nd | nd | 146±0.30b | nd |
TAC (mg AAE/g) | 40.3±2.04b | 74.5±2.76a | 88.7±2.99a | 34.5±1.98b | 82.9±2.54a | 49.8±1.76b |
Reducing Power (%) | 96.1±2.10a | 17.5±1.35b | 17.1±1.89b | 25.3±1.79b | 91.2±2.66a | 91.6±2.78a |
DPPH activity scavenging (%) | 28.6±0.31a | 30.1±0.23a | 30.1±0.16a | 1.09±0.54c | 4.8±1.17b | 6.2±0.43a |
Total ORAC value (mmol TE/100 g) | 12.2±0.67 | 17.4±1.02 | 19.49±0.97 | 2.75±0.18 | 20.2±0.66 | 21.9±1.87 |
Data are reported as means±SD with experiments performed in triplicate. GAE: gallic acid equivalent; CE: catechin equivalent; TE: trolox; nd: not determined. DPPH: 1,1-diphenyl-2-picrylhydrazyl assay for antiradical activity; ORAC: oxygen reactive antioxidant capacity. Antioxidant properties results were evaluated at 1.0 mg/mL concentration. TAC results are reported as ascorbic acid equivalent (AAE). Different letters indicate significant differences between different extracts of each residue at the same concentration (P<0.05, ANOVA).