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. Author manuscript; available in PMC: 2018 Mar 16.
Published in final edited form as: Cell. 2016 Oct 27;167(4):1014–1027.e12. doi: 10.1016/j.cell.2016.10.011

Figure 7. Kinetochore Model.

Figure 7

(A) Schematic cross section through a point-centromere kinetochore, illustrating the dimensional expansion from a centromeric nucleosome (bottom) to a microtubule (top). Various conserved molecular complexes are labeled and color-coded. MIND occupies a central position in the hierarchy extending from nucleosome to Ndc80C/DASH.

(B) Surface rendering of a 3-D view similar to the cross section in (A), but omitting the nucleosome-proximal components. The choice of a sixfold structure is arbitrary -- EM images appear to show 5,6, and 7 (Gonen et al, 2012). We have use head I - head II interactions seen in all our crystal forms to construct this model; most of its features do not depend on assuming that those particular contacts are present.

(C) Comparison of kinetochore model with experimental data. EM images adapted from Macmillan Publisher Ltd: [Nature Structural & Molecular Biology] (Gonen et al., 2012), copyright (2012). End-on view of the surface rendering, with nucleosome-proximal components omitted along with the microtubule and DASH, shows proposed head I to head II interactions that generate the central core. Perspective view on the right includes DASH and the microtubule from (B).