REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Dsn1560-572 QQLLKGLSLSFSK	Tufts University Core Facility	N/A
FITC-Dsn1560-572 FITC-AHA-QQLLKGLSLSFSK	Tufts University Core Facility	N/A
Mtw1230-262 KDFRTRYIDIRTNNVLRKLGLLGDKEDEKQSAK	Tufts University Core Facility	N/A
FITC-Mtw1230-262 FITC-AHA-KDFRTRYIDIRTNNVLRKLGLLGDKEDEKQSAK	Tufts University Core Facility	N/A
Mtw1274-289 SIDIEEPQLDLLDDVL	Tufts University Core Facility	N/A
FITC-Mtw1274-289 FITC-AHA- SIDIEEPQLDLLDDVL	Tufts University Core Facility	N/A
FITC-Mif21-41 MDYMNLGVKSRKTGLTVNKTVQKDEYSMENLNDFFKDEQDS	Tufts University Core Facility	N/A
FITC-Ame11-25 MDALKQRHLKLLYRQRGSASRTIDY	Tufts University Core Facility	N/A
PEG 550 MME	Hampton Research	HR2-611
PGA-LM	Molecular Dimensions	MD2-250-108
Ethylmercurithiosalicylic acid, sodium salt Heavy Atom Screen M1	Hampton Research	HR2-448
L(+)-Selenomethionine	Acros Organics	3211-76-5
PEG 8000	Sigma	81268-1kg
Tantalum Cluster Derivatization Kit	Jena Bioscience	PK-103
PEG 3000	Rigaku	1008057
Deposited Data
Atomic coordinates, MIND-C1 structure	Protein Data Bank	PDB: 5T58
Atomic coordinates, MN-C2 structure	Protein Data Bank	PDB: 5T51
Atomic coordinates, MN-C2-Mif21-41 structure	Protein Data Bank	PDB: 5T59
Atomic coordinates, Spc24/Spc25-Dsn1560-572 structure	Protein Data Bank	PDB: 5T6J
Experimental Models: Organisms/Strains
E. coli Rosetta 2 (DE3)	EMD	#71400
Recombinant DNA
pLIC_M42 H6-TEV- *MIND-C1	This study	N/A
pLIC_M147 H6-TEV- *MIND-C1:D205	This study	N/A
pLIC_M147_mut2 H6-TEV- *MIND-C1:D205-2D	This study	N/A
pLIC_M142 H6-TEV- *MIND-C1:D367	This study	N/A
pLIC_M89 H6-TEV- *head I MN-C2	This study	N/A
pLIC_M89_mut4 H6-TEV- *head I Site II mut.  Mtw1 N32A/Y36A/E73A/D77A	This study	N/A
pLIC_M89_mut5 H6-TEV- *head I Site III mut  Nnf1 E76A/E83A	This study	N/A
pLIC_M110 H6-TEV- *head II D256	This study	N/A
pLIC_M100 H6-TEV- *head II D230	This study	N/A
pLIC_M136 H6-TEV- *head II D205	This study	N/A
pLIC_M136_mut2 H6-TEV- *head II D205-2D	This study	N/A
pLIC_M107 H6-TEV- *head II D230L	This study	N/A
pLIC_Mf6 H6-MBP-TEV- *Mif2-FL	This study	N/A
pLIC_Mf3 H6-MBP-TEV- *Mif2 (1-41)	This study	N/A
pLIC_C6 H6-GST-TEV- *Ame1 (1-24)	This study	N/A
pLIC_S08 H6-TEV- *Spc24/Spc25	This study	N/A
pET30b-VHH3- *nanobody	This study	N/A
Software and Algorithms
XDS	(Kabsch, 2010)	xds.mpimfheidelberg.mpg.de
SHELXD	(Sheldrick, 2010)	shelx.uniac.gwdg.de/SHELX/
SHARP	(Vonrhein, 2007)	www.globalphasing.com/sharp/
RESOLVE	(Terwilliger, 2000)	https://solve.lanl.gov/Resolve/resolve.html
PHASER	(McCoy et al., 2007)	wwwstructmed.cimr.cam.ac.uk/phaser_obsolete/
PyMol	Schrödinger, LLC	www.pymol.org
DMMULTI	(Cowtan, 1994)	www.ccp4.ac.uk/html/dmmulti.html
PHENIX	(Adams et al., 2010)	www.phenix-online.org
O	(Jones, 1991)	Xray.bmc.uu.se/alwyn/A-Z_of_O/AZ_frameset.html
anisotropy server	(Strong, 2006)	https://services.mbi.ucla.edu/anisoscale/
Secondary structure prediction	(Yachdav et al., 2014)	www.predictprotein.org
MolProbity	(Chen et al., 2010)	Molprobity.biochem.duke.edu
Coot	(Emsley et al., 2010)	www2.mrclmb.cam.ac.uk/personal/pemsley/coot/
Prism7	GraphPad	www.graphpad.com
T-Coffee	(Notredame, 2000)	tcoffee.crg.cat
matplotlib	(Hunter, 2007)	matplotlib.org
ESPript	(Robert and Gouet, 2014)	espript.ibcp.fr/ESPript/ESPript/