Figure 2.

DHODH Exhibits Limited Binding of Mitochondrial Phospholipids

(A) Structures of the detergent LDAO and the three major phospholipid species in the human mitochondrial membrane.

(B) nESI-MS shows that binding of PC, PE, and CDL, can be preserved in the gas phase. CDL exhibits the most stable association with DHODH, while PC interactions are not well retained.

(C) Mass spectra of DHODH in the presence of increasing amounts of PE reveal a maximum number of lipid adducts for both apo- and holo-DHODH. Above a 20-fold excess (180 μM) of PE over DHODH, a maximum of three lipids are bound per protein.

(D) The DHODH-lipid complexes are less sensitive to DMSO-induced dissociation than the DHODH-FMN complexes (indicated by blue arrows). Spectra recorded without and with DMSO are normalized to the 14+ charge state of holo- and apo-DHODH, respectively.

See also Figure S2.