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. 2018 Feb 27;115(11):E2653–E2662. doi: 10.1073/pnas.1716224115

Fig. 3.

Fig. 3.

ERα–ERβ heterodimers recruit AF1ID in a ligand-dependent manner. (A) BRETFect analysis of ligand-dependent recruitment of CoA peptide (CoA) to ERα or ERβ homodimers or heterodimers. HEK293T cells expressing ERα/β-RLucII, ERα/β-mTFP1, and the CoA peptide fused to Venus or unfused controls (C, unfused ERα; +, unfused Venus) were treated with ligands (1 μM) for 45 min. (B) Delta BRET calculations from BRETFect assays in A. (C) BRETFect analysis of ligand-dependent recruitment of AF1ID to ER dimers. Cells expressing ERα/β-RLucII, ERα/β-mTFP1, and/or AF1ID fused to Topaz were treated as in A. (D) Delta BRET calculations from BRETFect assays in C. All graphs were prepared from at least three biological replicates, and error bars represent the SEM. Statistical significance was analyzed by ANOVA with a Bonferroni post hoc test. *P < 0.01, **P < 0.001.