Fig. 6.

Aggregation (A and B) (black solid lines) of mixed GDSs (900 μL) of both 3-Sulfo-Lac (0.1 mM) and 3-Lac (0.1 mM) to reach a final concentration 3-Sulfo-Lac (0.05 mM) and 3-Lac (0.05 mM) with Gal-4 (A) and Gal-8S (B) (2 mg·mL⁻¹, 100 μL) in PBS (pH 7.4). Aggregation (C–E) of GDSs with 3-Sulfo-Lac (0.05 mM, red lines), 3-Lac (0.05 mM, blue lines), and the sum curves (black dashed lines) of 3-Sulfo-Lac (0.05 mM) and 3-Lac (0.05 mM) were also indicated. Aggregation of GDSs (0.1 mM of 3-Sulfo-Lac, 900 μL) with mixed galectins (100 μL) of Gal-1 + Gal-4 (C), Gal-1 + Gal-4 (D), and Gal-4 + Gal-8S (E) with 1:1 ratio in PBS (pH 7.4). The aggregation (F and G) of GDS from 3-Sulfo-Lac with Gal-1 (green), Gal-4 (red), and Gal-8S (blue) (100 μL) at 1.0 mg·mL⁻¹. The sum curve (black dashed lines) of the combination of two relevant galectins was also indicated in each assay. Aggregation of GDSs (0.1 mM of 3-Sulfo-Lac, 900 μL) with incubation with Gal-8N (0 mg·mL⁻¹ to 2 mg·mL⁻¹, 100 μL) for 2 min, then Gal-4 (F) or Gal-8S (G) (1 mg·mL⁻¹, 100 μL) was added. Aggregation (H) of GDSs with Gal-3, Gal-3NT/8N, and Gal-8N (2.0 mg·mL⁻¹, 900 μL) in PBS (pH 7.4).