Table 2.

Measured transcellular CDF flux across Caco-2 monolayers, and CDF amounts in donor compartment and cells at 120 minutes following initial exposure to 5 μM CDFDA (Control), and with either 100 μM MK-571, glyceollin or genistein.

Condition	AB directional flux (pmol/min)		BA directional flux (pmol/min)		AB Donor (apical) CDF at 120 minutes (pmoles/well)		BA Donor (basolateral) CDF at 120 minutes (pmoles/well)		AB Intracellular CDF at 120 minutes (pmoles/well)		BA Intracellular CDF at 120 minutes (pmoles/well)
	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM
Control	0.28	0.004	0.81	0.018	101	14.5	143	23.2	29	2.2	83	6
MK-571	0.51	0.019	0.39	0.012	115	25.7	249	59.2	15	1.7	26	3.6
Glyceollin	0.63	0.041	0.53	0.041	95	2.8	187	9.9	15	1.3	30	9.4
Genistein	1.25	0.064	2.68	0.052	301	22.8	427	51.0	85	16.4	134	3.1

Flux was calculated from linear regression of the amount of CDF transported versus time from 30 to 120 minutes (zero-order and steady-state kinetic assumption). SEM refers to standard error of the mean. For each transport direction, there were 48 filters in control experiments and 9 filters for each agent. For a given transport direction, flux and cell content were significantly different from control in the presence of all three agents (p < 0.05) according to a 1-way ANOVA followed by Tukey’s test for multiple comparisons. For a given transport direction, donor amounts in the presence of either MK-571 or glyceollin were not significantly different from control; whereas, donor amounts were different for genistein.