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. Author manuscript; available in PMC: 2019 Mar 16.
Published in final edited form as: ACS Chem Biol. 2018 Feb 2;13(3):733–741. doi: 10.1021/acschembio.7b00879

Figure 1.

Figure 1

High throughput screening using in-cell NMR. (A) Screening for compounds that disrupt protein–protein interactions (PPIs) in vitro and in vivo produces hits with positive or negative responses. (B) Examining PPI targets by using in-cell NMR simultaneously affords the benefits of both in vitro and in vivo studies by providing amino acid residue specific information while the system remains in a physiologically relevant environment. Spectral data are analyzed by singular value decomposition (SVD) to ensure that the observed changes are real and not a result of an altered cellular environment.