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. Author manuscript; available in PMC: 2019 Mar 16.
Published in final edited form as: ACS Chem Biol. 2018 Feb 2;13(3):733–741. doi: 10.1021/acschembio.7b00879

Figure 2.

Figure 2

Screening by in-cell STINT-NMR. (A) Overlay of in-cell 1H–15N HSQC spectra of [U-15N]Pup overexpressed for 2 h (black) and [U-15N]Pup–Mpa complex (red) following 8 h of Mpa overexpression. Peaks labeled with an asterisk are metabolites. (B) Overlay of in-cell 1H–15N HSQC spectra of the [U-15N]Pup–Mpa complex containing 0.1% v/v DMSO (black) and 10 μM MTBA (red). (C) Overlay of in-cell 1H–15N HSQC spectra of the [U-15N]Pup–Mpa complex containing 0.1% v/v DMSO (black) and 10 μM MTBB (red). (D) Overlay of in-cell 1H–15N HSQC spectra of the [U-15N]Pup–Mpa complex containing 0.1% v/v DMSO (black) and 10 μM MTBC (red).