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Irradiation of photocaged L-azidohomoalanine (NPPOC-Aha) yields free Aha for ncAA in vitro incorporation. (a) Fluorescent tagging of fixed cells demonstrates similar fluorescent labeling (green) of Aha and irradiated NPPOC-Aha, but not L-methionine (Met) and unexposed NPPOC-Aha. (scale bar = 100 µM). (b) Metabolic labeling of cells in synthetic tissue is confined to regions near user-defined exposure (hashed area). Actin staining (red) remains uniform across sample (scale bar = 1 mm).
