Table 1.

Enzyme kinetic parameters

	Enzyme name	kcat (sec−1)	Km (µM)	kcat/Km (sec−1µM−1)	kobs @50 µMa (sec−1)	kobs @50 µMb (sec−1)
L-asparaginase activity	ErA-WT	207.5 ± 3.6	47.5 ± 3.5	4.37	118.9	145.8
	ErA-TM	261.2 ± 2.8	95.0 ± 3.5	2.75	79.6	56.4
	ErA-DM	169.8 ± 1.5	185.3 ± 5.5	0.92	22.4	23.3
	ErA-E63Q	186.8 ± 1.7	50.7 ± 2.0	3.68	112.7	135.9
	EcA-WT	44.4 ± 0.3	15.0 ± 0.5	2.96	41.3
	Enzyme name	kcat (sec−1)	Kmc(µM)	kcat/Kmc (sec−1µM−1)	kobs @0.5 mMa (sec−1)	kobs @0.5 mMb (sec−1)
L-glutaminase activity	ErA-WT	26.84 ± 0.26	360 ± 20	74.56 × 10−3	15.87	14.76
	ErA-TM	1.84 ± 0.11	47,460 ± 695	0.04 × 10−3	0.01	0.04
	ErA-DM	2.93 ± 0.03	15,800 ± 300	0.19 × 10−3	0.11	0.09
	ErA-E63Q	8.33 ± 0.16	3,860 ± 230	3.68 × 10−3	0.74	1.05
	EcA-WT	0.89 ± 0.01	1,380 ± 90	0.64 × 10−3	0.22
	Enzyme name	kobs[Asnphs] / kobs[Glnphs]d		kcat/Km (Asn) / kcat/Km (Gln)
Specificity	ErA-WT	6.6		58.6
	ErA-TM	8910		68,750
	ErA-DM	330		4,842
	ErA-E63Q	124.6		1,000
	EcA-WT	187.7		4,625

^ak_obs for enzymes without the SUMO tag

^bk_obs for enzymes with the SUMO tag

^cConcentrations are given in µM to facilitate comparison with the L-asparaginase data

^dk_obs for Asn@50µM, k_obs for Gln@500µM