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. 2001 Aug 28;98(19):10875–10879. doi: 10.1073/pnas.191224698

Figure 3.

Figure 3

Susceptibility of particles to proteolytic digestion. Particles assembled in buffer B (A), particles assembled in buffer B plus 5% rabbit reticulocyte lysate (B), immature HIV-1 virions (C), or particles assembled in buffer B plus 2 μM IP5 (D) were digested with HIV-1 PR (lanes 2) or with trypsin (lanes 3). Digestion products were examined by SDS/PAGE and immunoblotting with antibodies against HIV-1 CA protein. (E) N-terminal sequence of the trypsin-resistant fragment. The ≈26-kDa band in lane 3 was a mixture of molecules with zero, one, or two N-terminal lysines. The first lysine is residue 112 of Gag.