Fig. 2.

Phosphorylation of tau at Thr205 activates the nuclear Ca²⁺-CaMKIV pathway. A, B HEK293 cells were transfected with DsRed2-N1-phospho-mimic (T205E) or non-phospho-mimic (T205A) mutant tau plasmids for 24 h and then the nuclear fraction was prepared for Western blotting. An increased phosphorylation level of CaMKIV was detected in the nuclear fraction of T205E-transfected cells compared with T205A. C An increased basal [Ca²⁺] was detected in the nuclear fraction of T205E-transfected cells compared with T205A (>100 cells). Data are expressed as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001 vs T205A.