Fig. 3.

Phosphorylation of tau at Ser214 does not affect nuclear Ca²⁺ and CaMKIV activity. A, B HEK293 cells were transfected with DsRed2-N1-phospho-mimic (S214E) or non-phospho-mimic (S214A) mutant tau plasmids for 24 h and then the nuclear fraction was prepared for Western blotting. Compared with S214A, expression of S214E did not significantly change the levels of total CaMKIV and pCaMKIV in the nuclear fraction. C Compared with S214A, expression of S214E did not change the level of nuclear Ca²⁺. Data are expressed as mean ± SEM.