Fig. 5.

Inhibition of CaMKIV abolishes OA-induced tau hyperphosphorylation in the nuclear fraction. A, B HEK293 cells with stable expression of human wild-type tau (293Tau) were transfected with CaMBP4 plasmid or vector for 24 h, and a decreased level of pCaMKIV in the nuclear fraction of the CaMBP4-trasfected cells was detected by Western blotting (**P < 0.01 vs Vec). C, D HEK293 cells with stable expression of human wild-type tau were transfected with CaMBP4 or the vector for 24 h, and then treated with OA or DMSO for 12 h. CaMBP4 expression reduced the OA-induced tau hyperphosphorylation at Thr205, Ser214, Thr213, Ser396, and Ser404 in the nuclear fraction. Tau-5 probes total tau proteins. Data are expressed as mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001 vs Vec + DMSO; ^# P < 0.05, ^## P < 0.01 vs Vec + OA.