Fig. 1.

Perifosine pretreatment decreases Akt and S6 activation after KA-induced acute seizures. A KA-induced seizures result in activation of the mTOR pathway. Representative western blots showing that phospho-Akt (p-Akt) and phospho-S6 (p-S6) expression in the hippocampus increased within 1 h of the onset of KA-induced seizures in perifosine-pretreated (Peri + KA) and vehicle-pretreated (Veh + KA) rats. Vehicle-treated rats that did not receive KA (Veh + KA) served as an additional control. B Quantitative summary of p-Akt/Akt and p-S6/S6 at 1 h (*P < 0.05, vs Veh + NS group, # P < 0.05 vs Veh + KA group, n = 6 rats/group).