Fig. 3.

Effects of GluN2A-N447K on Mg²⁺ and Zn²⁺ sensitivity of NMDARs. A Representative evoked current traces of wild-type and mutant NMDARs at different holding potentials (current scale bar, 500 pA; time scale bar, 2 s). B Mg²⁺ current-voltage (I-V) relationship of GluN1/GluN2A-WT (black) and GluN1/GluN2A-N447K (red) NMDARs. C Representative current traces of wild-type and mutant NMDARs induced by co-agonists in the presence of 300 μmol/L Mg²⁺. Other doses of Mg²⁺ were applied in the same way (current scale bar, 500 pA; time scale bar, 1 s). D Mg²⁺ concentration-response curves reveal a decreased Mg²⁺ block for the GluN1/GluN2A-N447K NMDARs. E Representative evoked current traces of wild-type and mutant NMDARs inhibited by 1000 nmol/L Zn²⁺. Other doses of Zn²⁺ were applied in the same way (current scale bar, 500 pA; time scale bar, 1 s). F Zn²⁺ concentration-response curves show that Zn²⁺ inhibition of mutant NMDARs is undistinguishable from that of the WT.