Fig. 3.

Myt1L promotes OPC differentiation in vitro. A QPCR analysis of Myt1L mRNA and MBP mRNA in purified OPCs transfected with overexpressing virus (Myt1L-OE) and control virus. B, C Western blot analysis of MBP and Myt1L protein levels. D Representative images of anti-MBP immunocytofluorescence staining of OPCs transfected with Myt1L-OE. E Quantification of MBP⁺/GFP⁺ cells as in D. F QPCR analysis of Myt1L and MBP mRNAs in purified OPCs transfected with interfering virus (Myt1L-RNAi). G, H Western blot analysis of MBP protein levels. I Representative images of anti-MBP immunocytofluorescence staining of OPCs transfected with Myt1L-RNAi virus. J Quantification of MBP⁺/GFP⁺ cells as in I. Transfected cells were all cultured for 72 h in differentiation medium before analysis. *P <0.05, **P <0.01, Student’s t-test; n = 3 independent experiments; scale bars, 20 μm.