Fig. 4.

Myt1L does not affect OPC proliferation, survival, and migration. A, B Representative images showing BrdU immunocytofluorescence staining (red) in Myt1L-OE (A) and Myt1L-RNAi (B) virus-transfected OPCs (green). C, D Percentages of BrdU⁺/GFP⁺ cells among all GFP⁺ cells as in A and B. Data are normalized to control. E, F Representative images showing caspase3 immunocytofluorescence staining (red) in Myt1L-OE (E) and Myt1L-RNAi (F) virus-transfected OPCs (green). G, H Percentages of caspase3⁺/GFP⁺ cells among all GFP⁺ cells as in E and F. Data are normalized to control; n = 3 independent experiments. I, J Representative transwell assays visualizing migration of Myt1L-OE (I) and Myt1L-RNAi (J) virus-transfected OPCs. K, L Quantification of migrating cells as in I and J. Data are normalized to control. Student’s t-test: n = 5 independent experiments; scale bars, 20 μm.