Table 1.
Properties and kinetic constants of native fish trypsins
| Species | Molecular weight (kDa) | Optimum temperature (°C) | pH | Substrate assayed | Km (mM) | Kcat (s−1) | kcat Km−1 (s−1 mM−1) | References |
|---|---|---|---|---|---|---|---|---|
| Oil sardine (Sardinella longiceps) | 24 | 60 | 8 | BAPNA | 0.0206 | 1.19 | 57.76 | Khandagale et al. (2017) |
| Atlantic cod (Gadus morhua) trypsin X -variant 7 | 25 | 65 | 8.5–11 | BAPNA | 3.9 | 13.0 | 3.5 | Stefansson et al. (2017) |
| CBZ-FR-pNA | 0.050 | 25.9 | 520 | |||||
| CBZ-GPR-pNA | 0.008 | 64.9 | 8210 | |||||
| Golden grey mullet (Liza aurata) | 23 | 50 | 10 | BAPNA | 0.43 | 4.04 | 9.39 | Bkhairia et al. (2016) |
| Lophiosilurus alexandri | 24 | 50 | 9 | BAPNA | 0.517 | 5 | 9.67 | Dos Santos et al. (2016) |
| Unicorn leatherjacket (Aluterus monoceros) | 23.5 | 55 | 8 | BAPNA | – | – | – | Zamani and Benjakul (2016) |
| Cirrhinus mrigala | 21.7 | 30–40 | 7.6 | BAPNA | 0.0672 | 6.17 | 92.09 | Khangembam and Chakrabarti (2015) |
| Thornback ray (Raja clavata) | – | 50 | 8 | CASEIN | – | – | – | Lassoued et al. (2015) |
| Scorpionfish (Scorpaena scrofa) | – | 55 | 10 | CASEIN | – | – | – | Younes et al. (2015) |
| Pacific saury (Cololabis saira) | 24 | 55 | 8.5 | TAME | 0.19 | 210 | 1105 | Klomklao et al. (2014) |
| Common kilka (Clupeonella cultriventris caspia) | 23.2 | 60 | 8 | BAPNA | 0.10 | 7.93 | 79.3 | Zamani et al. (2014) |
| – | – | – | TAME | 0.07 | 28.5 | 407.1 | ||
| Crevalle jack (Caranx hippos) | 27.5 | 50 | 8 | BAPNA | 0.689 | 6.9 | 10 | Costa et al. (2013) |
| Catshark (Scyliorhinus canicula) | 28 | 55 | 8 | BAPNA | 0.104 | 0.23 | 2.21 | Blanco et al. (2014) |
| Indian mackerel (Rastrelliger kanagurta) | 26 | 50 | 9 | BAPNA | 0.430 | 0.77 | 1.78 | Khandagale et al. (2013) |
| Sailfin Catfish (Pterygoplichthys disjunctivus) | 27.5 | 40 | 9.5 | BAPNA | 0.13 | 1.46 | 11.24 | Villalba-Villalba et al. (2013) |
| Nile Tilapia (Oreochromis niloticus) genetically improved | 28 | 60 | 8 | TAME | 0.036 | 152 | 4242 | Zhou et al. (2013) |
| Flatfish (Paralichthys olivaceus) | 29.6 | 70 | 7.5 | BAPNA | 0.017 | 0.024 | 1.422 | Kim and Jeong (2013) |
| Carp (Catla catla) | 20.2 | 40 | 7 | BAPNA | 0.062 | 19.23 | 310.16 | Khangembam et al. (2012) |
| Goby (Zosterisessor ophiocephalus) | 23.2 | 60 | 9 | BAPNA | 0.312 | 2.03 | 6.51 | Nasri et al. (2012) |
| Nile tilapia (Oreochromis niloticus L.) | 22.39 | 55–60 | 9 | BAPNA | 0.16 | 23.8 | 238 | Unajak et al. (2012) |
| Piracucu (Arapaima gigas) | 28.0 | 65 | 9 | BAPNA | 0.47 | 1.33 | 2.82 | Freitas-Júnior et al. (2012) |
| Tropical sierra (Scomberomorus sierra) | 25.4 | 60 | 9 | BAPNA | – | – | – | Valdez-Melchor et al. (2013) |
| Japanese sea bass (Lateolabrax japonicus) | 21.0 (trypsin A) 21.5 (trypsin B) |
40 | 9 | Boc-Phe-Ser-Arg-MCA | 1.12 0.70 |
72.08 67.79 |
64.16 92.55 |
Cai et al. (2011) |
| Silver mojarra (Diapterus rhombeus) | 26.5 | 50–55 | 8.5 | BAPNA | 0.266 | 0.93 | 3.48 | Silva et al. (2011) |
| Hybrid catfish (Clarias macrocephalus/Clarias gariepinus) | 24 | 60 | 8 | TAME | 0.30 | 92.1 | 307 | Klomklao et al. (2011) |
| Atlantic cod (Gadus morhua) | 24.0 (trypsin I) | – | 8 | CBZ-Gly-Pro-Arg-pNA | 0.007 | 53.1 | 7590 | Stefansson et al. (2010) |
| Smooth hound (Mustelus mustelus) | 24 | 50 | 8.5 | BAPNA TAME |
0.387 0.156 |
2.62 59.15 |
6.77 379.16 |
Bougatef et al. (2010) |
| Skipjack tuna (Katsuwonus pelamis) | 24 | 55 (trypsin A) 60 (trypsin B) |
9 | TAME | 0.22 0.31 |
82.5 69.5 |
266.13 315.91 |
Klomklao et al. (2009b) |
| Pacific cod (Gadus macrocephalus) | 24 | 50 | 8 | TAME | – | – | – | Fuchise et al. (2009) |
| Saffron cod (Eleginus gracilis) | 24 | 50 | 8 | TAME | – | – | – | Fuchise et al. (2009) |
| Sardinelle (Sardinella aurita) | 24 | 55 | 8 | BAPNA | 1.67 | 3.87 | 2.31 | Ben Khaled et al. (2008) |
| Mandarin fish (Siniperca chuatsi) | 21.0 (trypsin A) 21.5 (trypsin B) |
35 (A) 40 (B) |
8.5 | Boc-Phe-Ser-Arg-MCA | 0.00218 0.00188 |
81.6 111.3 |
37431.19 59202.12 |
Lu et al. (2008) |
| Atlantic wolffish (Anarhichas lupus) | – | > 80 | 9 | BAPNA | 0.55 0.56 |
– | – | Desrosiers et al. (2008) |
| Walleye pollock (Theragra chalcogramma) | 24 | 50 | 8 | TAME | – | – | – | Kishimura et al. (2008) |
| Atlantic bonito (Sarda sarda) | 29 | 65 | 9 | BAPNA | – | – | – | Klomklao et al. (2007b) |
| Skipjack tuna (Katsuwonus pelamis) | 24 | 60 | 8.5 | TAME | 0.29 (trypsin A) 0.20 (trypsin B) 0.11 (trypsin C) |
114 61.5 57.1 |
393.10 307.50 519.09 |
Klomklao et al. (2007c) |
| Grass carp (Ctenopharyngodon idellus) | 30.7 (trypsin A) 26.4 (trypsin B) | 38.5 44.0 |
8.0 8.5 |
BAEE | 0.0212 0.0317 |
33.3 55.0 |
1572.32 1735.01 |
Liu et al. (2007) |
| Hoki fish (Macruronus novaezelandiae) | 26 | 60 | 9 | BAPNA TAME |
0.06 2.08 |
0.33 19.00 |
5.50 9.13 |
Shi et al. (2007) |
| Spotted goatfish (Pseudupeneus maculatus) | 24.5 | 55 | 9 | BAPNA | 1.82 | – | – | Souza et al. (2007) |
| True sardine (Sardinops melanostictus) | 24 | 60 | 8 | TAME | – | – | – | Kishimura et al. (2006a) |
| Arabesque greenling (Pleurogrammus azonus) | 24 | 50 | 8 | TAME | – | – | – | Kishimura et al. (2006a) |
| Monterey sardine (Sardinops Sagax Caerulea) | 25 | 50 | 8 | BAPNA | 0.051 | 2.12 | 41.0 | Castillo-Yáñez et al. (2005) |
| Anchoa (Engraulis japonicus) | 24.5 (trypsin II) |
60 | 9.5 | BAEE BAPNA |
0.014 0.033 |
42.09 3.70 |
3006 112 |
Ahsan and Watabe (2001) |
| Anchoa (Engraulis japonicus) | 24.5 (trypsin I) |
60 | 9.5 | BAEE BAPNA |
0.031 0.037 |
34.56 1.94 |
1114 52 |
Ahsan and Watabe (2001) |