Table 3.
Recombinant fish trypsins obtained in different expression systems
| Species | Enzyme | Expression vector | Expression system | Yield | Molecular weight (kDa) | Substrates assayed | References |
|---|---|---|---|---|---|---|---|
| Medaka (Oryzias latipes) | Trypsin | pET30a (Novagen, Madison, WI) | E. coli | – | 38 Trypsinogen fusion protein 24 |
Boc-Gln-Ala Arg-MCA | Rajapakse et al. (2014) |
| Engraulis japonicus (Anchoa) | Trypsin | pETBlue1 |
E. coli
Rosetta (DE3) pLacI Tuner (DE3) pLacI |
12 mg L−1 | 24 | BAPNA | Ahsan et al. (2005) |
| Tautoga americana/cunner-fish (Tautogolabrus adspersus) | Trypsin | pPICZ y pPICZα |
Pichia pastoris
Cepa X-33 (mut+his+) |
Low (NE) | 45 (glycosylation) 35.4 (rCFT fused to the myc epitope) |
BAPNA | Macouzet et al. (2005) |
| Atlantic cod (Gadus morhua) | Trypsin Y | pThioHis A | E. coli TOP10 | 0.15 mg L−1 (Mono Q) | 40 (HP-thioredoxin-trypsin Y) 25 |
N-CBZ-Gly-Pro-Arg-pNA Suc-Ala-Ala-Pro-Phe-pNA |
Pálsdóttir and Gudmundsdóttir (2007) |
| Atlantic cod (Gadus morhua) | Trypsin Y | pPiczα A |
Pichia pastoris
Cepa X-33 (mut+his +) |
300 mg L−1 (Fermentor) | 30 (r-trypsin Y-HisMyc polypeptide) 27 |
Suc-Ala-Ala-Pro-Arg-p-nitroanilide N-CBZ-Gly-Pro-Arg-pNA Suc-Ala-Ala-Pro-Phe-pNA |
Palsdottir and Gudmundsdottir (2004) |
| Atlantic cod (Gadus morhua) | Trypsin I (cold-adapted) | pThioHisA | E. coli Top 10 | 0.038 mg L−1 (Mono Q) 300 mg L−1 (Fermentor) |
39 (HP-thioredoxin-trypsin I) 24 (Native trypsin I) |
N-CBZ-Gly-Pro-Arg-pNA | (Jónsdóttir et al. 2004) |