Fig. 3.
Photomicrograph and results of the analysis of Brillouin and Raman spectra of C. albicans on an aluminum substrate [26, 34]. The green line denotes a 20-μm-long region where Brillouin and Raman spectra were collected, using a 1-μm step size. Righthand panels Brillouin intensity I0, frequency shift and linewidth, proportional to thickness, hardness and viscosity, respectively. Lefthand panels Raman integrated intensity of the OH region (3100–3800 cm−1), and of amide I + EPS constituents + cytochrome c (1515–1750 cm−1) normalized to the intensity of the CH2 and CH3 stretching region (2800–3100 cm−1)