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. 2018 Mar 16;8:4733. doi: 10.1038/s41598-018-23042-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differentially expressed genes in stretched neonatal rat ventricular myocytes. (A,B) Numbers of differentially expressed genes in response to 1–48 hours of cyclic mechanical stretch in cultured cardiac myocytes with (A) 2-fold difference and (B) 1.5 fold difference. Black columns, upregulated genes; white columns downregulated genes, n = 5 in all groups. (C–I) Venn diagrams indicate the overlap of the genes that were significantly (C) upregulated (>2-fold) or (D) downregulated (<2-fold) and (E) upregulated (>1.5-fold) or (F) downregulated (<1.5-fold) after 1, 4 or 12 hours of stretch as compared to controls. A Venn diagram indicating the overlap of the genes that were significantly (G) upregulated (>2-fold) or (H) downregulated (<2-fold) and (I) upregulated (>1.5-fold) or (J) downregulated (<1.5-fold) after 24 or 48 hours of stretch compared to controls.