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. 2018 Mar 16;8:4722. doi: 10.1038/s41598-018-22971-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of ALK5 leads to an enrichment in cobblestone-shaped cells. Phase contrast figures of control DMSO (a,b) and SD-208 (c,d) treated explants. Boxed areas in panels (a) and (c) are magnified in panels (b) and (d) respectively. (e) Quantification of the relative amount of cobblestone-shaped cells in the outgrowths. (f) The total number of migrating cells normalized to the explant area (presented per 100 µm² explant area). The individual datapoints, the mean and standard deviation are presented in panels (e) and (f). The explants (ex) is delineated by black dashes. The area of cellular outgrowth is delineated by a white loop.