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. 2018 Mar 16;8:4722. doi: 10.1038/s41598-018-22971-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of ALK5 leads to a relative increase in epicardial and endothelial cells. (a–t) 10 pairs of somite matched AVC explants treated with either DMSO or SD-208 in DMSO were stained for GFP, WT1 and αSMA. Boxed areas in panels (a), (f), (k) and (p) are magnified in respectively (b–d), (g–i), (l–n) and (q–s) and presented as alternating combinations of 2 channels in magenta and green. Individual confocal Z-planes are compared with the corresponding phase contrast images (e,j,o and t). The explant (ex) is delineated by a white loop. The area of cellular outgrowth in panels (a), (f), (k) and (p) is delineated by a gray loop. Upon SD-208 treatment, the observed monolayers are either epicardium only (f–j), endothelium only (p–t) or mixed epicardium and endothelium (k–o).