Fig. 1. The results of validation experiments.

FSH stimulation for 1 h activated JNK pathway in the GCT cell line COV434 as evidenced by a robust increase in phospho-c-Jun^Ser63 level. Treatment with pharmacological JNK inhibitors SP600125 and AS601245 for 30 min at the indicated concentrations inhibited the phosphorylation of c-Jun in a dose-dependent manner in both western blot (a) and immunofluorescence staining (b). Knockdown efficiency of JNK siRNA was confirmed by decreased expression of total protein levels of JNK on western blot (c). Kinase assay confirmed that JNK activity was substantially inhibited with pharmacological inhibitors (50 μM) and siRNA (50 nM) (d). Scale bar: 50 µm