Fig. 5. The effect of JNK inhibition on cell cycle progression of COV434 cells.

Cell cycle progression at G1/S and G2/M before and after JNK inhibition was analyzed by EdU uptake and flow cytometry with the expression of the cyclins on western blot, respectively. JNK inhibition at G1/S was associated with reduced EdU uptake (a, b). The cells accumulated and failed to exit mitosis when JNK was inhibited at G2/M transition as quantitatively shown by the fraction of the cells by flow cytometric analysis (c) and the absence of de-phosphorylation of p-cdc2^Thy15 and degradation of cyclins A and B on western blot analysis (d). Scale bar: 100 µm