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. 2018 Mar 16;8:4739. doi: 10.1038/s41598-018-22991-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Diagrams for differentially expressed genes (DEGs) on osteogenic-induced (IN) and non-induced (CT) cells. Heatmaps of the DEGs are shown for the polysomal RNA fraction (A) and for the total RNA fraction (B). Data are displayed with different samples in the rows (three biological replicates for IN and for CT) and color intensities represent gene upregulation (red) and downregulation (yellow). (C) Scatterplot of log-10 RPKM values representing each gene in the polysomal fraction (median of reads per kilobase per million mapped reads from all patients for CT and IN samples). (D) Venn diagrams picturing set relationships between DEGs in the polysomal and total RNA fractions for upregulated genes (Up; left) and for downregulated genes (Down; right).