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. 2018 Mar 16;8:4739. doi: 10.1038/s41598-018-22991-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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hASCs need 21 days of in vitro induction to become osteoblasts. hASCs (A) or BM-MSC (B) were maintained in differentiation-inducing conditions for different periods of time and allowed to complete osteogenic differentiation (total of 21 days). The graphs represent the means (with standard deviations) of the relative fluorescence units (RFU) for OsteoImage^TM staining in each time period. (C) Representative images of induced hASCs after 24 h to 21 days of differentiation period. Green fluorescence staining of the hydroxyapatite portion of the mineralized matrix on cell cultures after 21 days. ***denotes p < 0.001.