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. 2018 Mar 16;8:4698. doi: 10.1038/s41598-018-23090-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electrophoretic profile of Rhipicephalus microplus ovary proteins. The protein profile of ovaries from partially engorged female groups (PEF-10, -16, -24, -35, -53, -84, -189, and -270) and fully engorged females (FEF) groups, which were categorised according to post-detachment day (D1, D2, D3 and D4), were analysed by SDS-PAGE. Approximately 50 µg of total protein was resolved on 10% gels and stained with Coomassie blue. MW: molecular weight standards, indicated in kDa on the right; Vt: purified Rhipicephalus microplus vitellin. Numbers of ticks composing each group are: PEF-10 (n = 10), PEF-16 (n = 9), PEF-24 (n = 28), PEF-35 (n = 23), PEF-53 (n = 8), PEF-84 (n = 9), PEF-189 (n = 5), PEF-270 (n = 5). FEF groups D1, D2, D3, and D4 were composed by ovaries from 5 ticks. This image is representative of two independent SDS-PAGE experiments.