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. 2018 Mar 16;8:4698. doi: 10.1038/s41598-018-23090-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative proteomic analysis of Rhipicephalus microplus partially engorged females (PEF) ovaries. Dimethyl labelling quantification of ovary proteins in PEF groups -24, -35, -53, -84, -189 and -270 was performed. Area chart shows the variation in protein levels (y axis) as a function of ovary maturation (z axis). The x axis shows the categories in which proteins were clustered. The data from each group was normalised relative to the group PEF-24. Numbers of ticks composing each group are: PEF-10 (n = 10), PEF-16 (n = 9), PEF-24 (n = 28), PEF-35 (n = 23), PEF-53 (n = 8), PEF-84 (n = 9), PEF-189 (n = 5), PEF-270 (n = 5).