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. 2018 Mar 16;8:4698. doi: 10.1038/s41598-018-23090-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vitellin (Vt) and vitellin degrading cysteine endopeptidase (VTDCE) in Rhipicephalus microplus ovaries analysed by western blot. Protein extracts from partially (PEF) and fully engorged females (FEF) ovaries were probed with rabbit antibodies (a) anti-Vt and (b) anti-VTDCE. PEF groups: -10, -16, -24, -35, -53, -84, -189, -270; FEF groups: -D1, -D2, -D3 and -D4. Gaps were created in the images to delineate the stages of ovary maturation, but both parts are from the same gel. This image is representative of two (for VTDCE) and four (for Vt) independent western blot. Numbers of ticks composing each group are: PEF-10 (n = 10), PEF-16 (n = 9), PEF-24 (n = 28), PEF-35 (n = 23), PEF-53 (n = 8), PEF-84 (n = 9), PEF-189 (n = 5), PEF-270 (n = 5). FEF groups D1, D2, D3, and D4 were composed by ovaries from 5 ticks. This image is representative of two (for VTDCE) and four (for Vt) independent western blot.