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. 2018 Mar 16;8:4698. doi: 10.1038/s41598-018-23090-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of proteins in selected metabolic pathways related to vitellogenesis. Dimethyl labelling quantification was performed in ovary extracts of partially engorged female (PEF) groups -24, -35, -53, -84, 189, -270. The ratio is the intensity detected for each peptide, relative to the internal standard (a protein pool of all ovary groups used in this study). The average ratio of a protein is calculated as the mean ratio of its peptides. The average ratio of each metabolic pathway presented in this graph was calculated from the average quantification (with standard deviation) of enzymes involved in each pathway (Supplementary Fig. S1). Numbers of ticks composing each group are: PEF-10 (n = 10), PEF-16 (n = 9), PEF-24 (n = 28), PEF-35 (n = 23), PEF-53 (n = 8), PEF-84 (n = 9), PEF-189 (n = 5), PEF-270 (n = 5).