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. 2018 Mar 16;9:1106. doi: 10.1038/s41467-018-03494-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Inhibition of PLK1 decreases the levels of Aurora B, CENP-A pS7 and Histone 3 pT3 in cells expressing APC-ΔC. a Scheme of the experimental procedure. APC-ΔC-expressing and control HCT116 cells were seeded on day 1, treated on day 2 either with 2 µM STLC or 100 nM BI6727 for 16 h and harvested on day 3. Cells were fixed, processed for immunofluorescence using antibodies for (b) Aurora B, (c) Histone 3 pT3 (H3 pT3), (d) CENPA pS7and ACA. Intensities were normalized to ACA and quantified. Scale bar, 5 µm. Values were calculated from at least 50 cells per treatment. (means ± s.d., n = 3, for each treatment). ***P < 0.001, Mann-Whitney U-test