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. 2018 Mar 16;8:4712. doi: 10.1038/s41598-018-23104-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ZFYVE28 gene expression is enriched in podocytes. (A) In human kidney, ZFYVE8 transcript shows strong enrichment in the glomerulus (GLOM) when compared with the rest of the kidney (ROK) as detected by conventional RT-PCR (left) and qPCR (right). 28 S gene was used as a loading control and nephrin to validate the purity of glomerular fractions. Values are expressed as mean ± SD of 3 samples. ***p < 0.001. The pictures of the original gels are shown in the Supplementary Figure 1. (B) In mouse kidney, ZFYVE28 transcript is highly enriched in the glomerulus as detected by conventional RT-PCR (left). Quantitative PCR shows a strong enrichment of ZFYVE28 in isolated mouse podocytes. Nephrin was used to validate the purity of podocytes. GAPDH was used as a loading control. (C) Immunofluorescence staining for ZFYVE28 (green) in human kidney shows strong immunoreactivity in glomeruli and no significant signal in extra-glomerular areas. (D) Double staining with podocyte foot process marker nephrin (red) shows nearly complete co-localization (yellow). No significant overlapping reactivity was detected with podocyte major process marker vimentin (red). DAPI (blue) was used as a nucleus marker. Scale bars in C and D: 50 μm.