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. 2018 Mar 16;8:4712. doi: 10.1038/s41598-018-23104-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of ZFYVE28 protein during human glomerulogenesis. (A) Immunofluorescence staining for ZFYVE28 (green) in human fetal kidney shows strong signal in developing glomeruli. Scale bar 50 μm. (B) S-shaped glomeruli show no staining for ZFYVE28 (green) or nephrin (red). At the capillary stage glomerulus ZFYVE28 co-localizes with nephrin at the basal aspects of pre-podocytes and between pre-podocytes (arrowheads). In maturing stage glomeruli, ZFYVE28 is detected at the basal side of developing podocytes. (C) Double labeling experiment with vimentin does not show co-localization during podocyte development. Scale bars 10 μm.