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. 2018 Mar 16;8:4662. doi: 10.1038/s41598-018-23112-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IL-10 expression of mouse peripheral CD8+ lymphocytes incubated with mouse embryo-derived EVs, or with anti-PIBF treated mouse embryo-derived EVs. Anti-CD8 labelled mouse spleen cells were incubated with embryo derived EVs, or with EVs that had been pre-treated with anti-PIBF antibody for 30 mins. Anti-rabbit IgG was used as an isotype control. Then the cells were labelled with anti- IL-10 antibody, and the number of IL-10 positive cells among the subpopulations was determined by flow cytometry. The bars represent the mean+/− SEM of 15 measurements. *p < 0.001.