Fig. 4.

Comparative Ty1-copia and Ty3-gypsy in situ hybridization analysis in rye, wheat and triticale after DAPI staining. Asterisk, DAPI image; double asterisk, DAPI image with superimposed location of the probe; somatic cell metaphase chromosomes. a–f Ty1-copia in situ hybridization. a Lanca wheat metaphase plate. b Wheat chromosome with 1RS translocation in common wheat cultivar Lanca. c, d Metaphase plates of rye cultivar Muro. e LMUR triticale metaphase plate, visible local density of retrotransposons in centromeres. f LMUR triticale metaphase plate, arrows indicate chromosome 1R satellites containing retrotransposons. g–j Ty3-gypsy in situ hybridization. g Metaphase plate of common wheat cultivar Lanca. h Metaphase plates of rye cultivar Muro. i, j LMUR triticale metaphase plates. Scale bar 5 µm