Fig. 2.

SORBS2 depletion promotes ovarian cancer metastasis. a Western blot analysis for SORBS2 in whole-cell lysate of A2780s cells expressing either of two independent shRNAs targeting SORBS2 or a control shRNA. b Representative photographs of peritoneal metastasis of A2780s and SKOV-3 ovarian cancer cells 4 weeks post-inoculation. c Box plot of number of metastatic nodules of tumors in the abdominal cavities. d Box plot of the ascites volumes collected from the abdominal cavities. e Kaplan-Meier analysis of mice in the orthotopic model generated by intrabursal injection of either A2780s or SKOV-3 ovarian cancer cells expressing either of two independent shRNAs targeting SORBS2 or a control shRNA. f Cell migration capacity of SORBS2-depleted A2780s and SKOV-3 ovarian cancer cells compared with control cells was assessed by transwell analysis. g Cell migration capacity of SORBS2-depleted A2780s and SKOV-3 ovarian cancer cells compared with control cells was assessed by wound healing analysis. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001