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. 2018 Mar 16;15:85. doi: 10.1186/s12974-018-1106-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Chronic exposure to TNFα impairs the immunosuppressive function of RPE cells. a Representative pictures of human monocytes (hMos, human hematopoietic transcription factor (PU1) staining, green) and RPE nuclei (Hoechst, blue) and b automated quantification of the number of human monocytes in a 24-h co-culture with RPE cells with or without previous 10-day pre-treatment with TNFα (at 0.8, 4, and 20 ng/ml) (n = 5/group, one way ANOVA versus control ***P < 0.0005)