Figure 2.

NPRL2 overexpression enhances CPT-11 sensitivity of CRC cells. The IC50 of CPT-11 was lower in cells transduced with NPRL2 than in cells transduced with empty vector (EV) (A). HCT116 cells treated with empty vector (EV) (with or without 25 μg/ml CPT-11) or with NPRL2 (with or without 25 μg/ml CPT-11) for an additional 24, 48 and 72 h, and cell viability was detected by a CCK8 assay (B). Results in bar graphs are represented as mean ±SD from three different triplicate experiments. * P<0.05; ** P<0.01.