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. 2018 Mar 9;24:1424–1433. doi: 10.12659/MSM.909186

Figure 4.

Figure 4

Overexpression enhances sensitivity to CPT-11 by increasing γ-H2AX expression in CRC cells. The protein levels of γ-H2AX in HCT116 cells treated with empty vector (EV), NPRL2, empty vector (EV) plus CPT-11 or NPRL2 plus CPT-11 for 24 h were evaluated by Western blotting. GADPH served as loading Control (A, B). γ-H2AX expression were examined in cells treated with empty vector (EV), NPRL2, empty vector (EV) + CPT-11 or NPRL2 + CPT-11 for 24 h by immunofluorescence staining (C). The bar graph summarizing nuclear foci distribution in the above four groups (D). Results in bar graphs are represented as mean ±SD from three different triplicate experiments. * P<0.05; ** P<0.01.