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. 2018 Mar 9;24:1424–1433. doi: 10.12659/MSM.909186

Figure 5.

Figure 5

NPRL2 overexpression enhances sensitivity to CPT-11 by inducing G2/M cell cycle arrest and activating the DNA damage checkpoint pathway in CRC cells. Cell cycle distribution in HCT116 cells treated with empty vector (EV) (with or without CPT-11) or with NPRL2 (with or without CPT-11) for 48 h (A). Bar graphs of HCT116 cells cycle distribution (B). HCT116 cells treated with an empty vector (EV), NPRL2, empty vector (EV) plus CPT-11 or NPRL2 plus CPT-11 were harvested at 48 h and then the expression of p-ATM (S1981), p-Chk2 (Thr68), Cdc25C, Cyclin B1 and Cdc2 were analyzed by Western blotting. GADPH served as loading Control (C). Results in bar graph are represented as mean ±SD from three different triplicate experiments. * P<0.05; ** P<0.01.