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. 2018 Mar 9;24:1424–1433. doi: 10.12659/MSM.909186

Figure 6.

Figure 6

NPRL2 overexpression improves CPT-11–induced inhibition of invasion and migration through the down-regulation of PI3K/AKT pathway-dependent MMP reduction. Changes of migratory potential were detected by wound-healing assay. The cells were pre-treated empty vector (EV) (with or without CPT-11) or with NPRL2 (with or without CPT-11) for 24 h (A). Transwell assay were done in HCT116 cells treated with empty vector (EV), NPRL2, empty vector (EV) plus CPT-11 or NPRL2 plus CPT-11 for 24 h (B). The protein levels of MMP2, MMP9, p-PI3K and p-AKT in HCT116 cells treated empty vector (EV) (with or without CPT-11) or with NPRL2 (with or without CPT-11) for 24 h were quantified by Western blotting (C, D). Results in bar graphs are represented as mean ± SD from three different triplicate experiments. * P<0.05; ** P<0.01.